Biosynthesis and Characterisation of Silver Nanoparticles using Leaf Extract of Terminalia Belerica for its Antimicrobial Activity
Keywords:
Nanotechnology, Green synthesis, Silver nanoparticles, Antimicrobial activityAbstract
In the current study, silver nanoparticles were synthesized using the ethanolic leaf extract of Terminalia belerica . Characterization was carried out using UV-Visible, FTIR, XRD, SEM and TEM. The formation of the AgNPs was confirmed through UV-Visible spectroscopy by the colour change. Based on the XRD pattern, the crystalline property of the AgNPs was established. The functional group present in the ethanolic leaf extract of Terminalia belerica is responsible for the reduction of the Ag+ ion which was studied through FTIR. From the SEM and TEM analysis, it was found that the formed nanoparticles are spherical in shape and nano in size. The biosynthesized AgNPs was evaluated for its antimicrobial activity against gram positive bacteria (S. aureus and L. bacillus) and gram negative bacteria (E. coli and P. aeruginosa) using disc diffusion and dilution method and it was found to exhibit potential antimicrobial activity.
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