Method Development and Validation for the Simultaneous Estimation of Paracetamol and Diclofenac by Green Spectroscopy in Tablet Dosage Form
Keywords:
Paracetamol, Diclofenac sodium, Simultaneous equation, Ethanol, UV SpectroscopyAbstract
A simple, rapid, accurate, precise, and economic spectrophotometric method for simultaneous estimation of paracetamol and diclofenacin tablet dosage form have been developed and validated.Paracetamol and diclofenac show absorbance maximums at 242 and 276nm respectively by using 7.4 phosphate buffers, so absorbance was measured at the same wavelengths for the estimation of paracetamol and Diclofenac. Absorbance is measured at 240.2 nm (Isoabsorptive point) and 258.4nm (λmax of diclofenac). Both drugs obey the Beer’sLambert's law in the concentration range of 10-30µg/mL. Methods are validated according to ICH guidelines and can be adopted for the routine analysis of Paracetamol and Diclofenac in tablet dosage form.
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