Simultaneous Estimation of Linagliptin and Metformin Hydrochloride in Bulk and Combined Tablet Dosage Form by UV-Spectrophotometric Methods
Keywords:
Linagliptin, Metformin hydrochloride, Simultaneous equation method, Second order derivative methodAbstract
Two simple, accurate, rapid and precise UV Spectrophotometric methods have been developed for simultaneous estimation of Linagliptin (LIN) and Metformin hydrochloride (MET) in combined tablet dosage form. The methods employed were (A) Simultaneous equation and (B) Second order derivative method. Method-A involves which involved measuring the absorbance values at 297 nmand 232nmof overlay spectrum of LIN &MET respectively. Method B is Second order derivative spectrophotometry, which involved measuring the absorbance values at 297 nm and 232nm of second derivative spectrum of LIN &MET respectively. In both the methods linearity was found in the concentration range of 0.5-2.5 μg/ml and 5-25 μg/ml respectively. Both the methods were found to be rapid, specific, precise and accurate. Hence these methods can be applied for routine analysis of Linagliptin and Metformin hydrochloride in combined dosage form without any interference by the excipients. The above methods are validated according to ICH guidelines.
References
Amar Gangadhar Zalte, Ravindranath Bhanudas Saudagar, Pramod Nanasaheb Katkade, (2016). Validated UV spectroscopic estimation of Linagliptin concentration in bulk and dosage forms. Volume - 9, Issue.
Amruta B L, Minal R G, Sawant S D. (2012). Simultaneous UV spectrophotometric method for estimation of Sitagliptin Phosphate and Metformin Hydrochloride in bulk and tablet dosage form. Der Pharma Chemical. 4(3): 854-9.
Dhabale P N, Seervi C R. (2010). Simultaneous UV Spectrophotometric Method for Estimation of Gliclazide and Metformine Hydrochloride in tablet dosage form. CODEN (USA) IJCRGG. 2(2):813-7.
John H B, John M B. (2004). Wilson & Gisvold’s textbook of Organic medicinal and Pharmaceutical Chemistry. 11th ed. p. 672.
Laurene L B, Jhon S L, Keith L P. (2006). Goodman and Gilman’s the pharmacological basis of Therapeutics. 11th ed. Mc Graw Hill, New York, U.S.A. p. 1638-39.
Maria C R, Mihaela I, Lavinia H, Elena I, Valentina A, Aurelian E R. et al. (2009). Constantin M. Validation of a HPLC method for the simultaneous analysis of Metformin and Gliclazide in human plasma. FARMACIA. 57(6):728-35.
Ramzia I E, Ehab F E, Bassam M A. (September, 2012). Liquid Chromatographic determination of Alogliptin in bulk and in its pharmaceutical preparation. Int J Biomed Sc,8( 3):215-8.
Sujana K, Swathi R G, Bhanu P M, Saheethi R M. (2010). Simultaneous estimation of Pioglitazone Hydrochloride and Metformin Hydrochloride using UV spectroscopic method in tablet dosage form. J Biomed Sci & Res. 2(2):110-5.
Wanjari M M, There A W, Tajne M R, Chopde C T, (Mar-Apr2008). Umathe S. N. Rapid and simple RPHPLC method for the estimation of Metformin in rat plasma. Indian J Pharm Sci.70(2):198-202.
