Invitro Thrombolytic Activity on Leaf Extract of Vitex Leucoxylon

Abstract

The study is regarded for standardization of leaf extract of Vitex leucoxylon, study focus on phytochemical investigations, isolation of flavonoids and evaluation for thrombolytic activity from various solvents. Pharmacognostical, phytochemical studies and in-vitro thrombolytic activity of leaves extract of Vitex leucoxylon was carried out by using in-Vitro model. The extracts were subjected to qualitative chemical analysis; chromatographic studies (TLC & HPTLC) were performed for flavonoids detection. The samples blood transferred to sterile microcentrifuge tubes incubated at 300C for 90mins. The serum removed completely and each tube having clot weighed to get clot weight. The serum with the clot added standard drug or plant extract. The tubes again incubated at 370 C for 45mins, the fluid obtained after clot lysis removed and tube again weighed. An alcoholic extract (20mg/100µl & 10mg/100µl) was evaluated in incubated blood. An attempt made to standardize leaf extract of Vitex leucoxylon, was successful and showed significant thrombolytic effect in comparison with thrombosis control in comparison with standard thrombolytic agent streptokinase (30000unit/100µl). Morphological study has provided a characteristic identity of leaf which have purplish pink/reddish pink colour, variable in-shape such as ovate, lanceolate, obtuse apex petiolate, and was found to be bitter in taste & mild flavour odour. And also determined the present of alkaloids, flavonoids, phenols, tannins, carbohydrates, saponins, glycosides. From the above-mentioned studies, it can be concluded that the Pharmacognostical standards generated will be useful for the proper identification of plant and also to differentiate it from its closely related species and adulterants. With the support of in vitro studies and phytochemical screening, the ethanolic extracts were showed more efficacies for thrombolytic activity.